Fluidics system features a true fixed-alignment flow cell that is gel-coupled to the collection optics to maximize detector signal. BD FACSDiva™ software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. The Cytometer Setup and Tracking (CS&T) feature of BD FACSDiva software establishes baseline settings and adjusts for instrument variability. The software reduces operator error, and ensures consistency of results by setting the signal time delay across the multiple laser beams and optimizing PMT voltages.

Working principle:Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths. The fluorescence can then be measured to determine the amount and type of cells present in a sample. A beam of laser light is directed at a hydrodynamically-focused stream of fluid that carries the cells. The suspended particles or cells, which may range in size from 0.2 to 150μm, pass through the beam of light and scatter the light beams. The fluorescently labelled cell components are excited by the laser and emit light at a longer wavelength than the light source. This is then detected by the detectors. The detectors therefore pick up a combination of scattered and fluorescent light. This data is then analyzed by a computer that is attached to the flow cytometer using BD FACSDiva software.

• Cell Sorting
• Cytokine assays
• Immune monitoring and Immune profiling
• Cell Proliferation Assays
• Immunophenotyping

• Quality control (QC) tracking capabilities in the software measure instrument settings and report on performance. Levey-Jennings plots help users understand instrument performance and identify maintenance issues.